Journal: PLoS ONE

Article Title: Long Term Expansion of Bone Marrow-Derived hMSCs on Novel Synthetic Microcarriers in Xeno-Free, Defined Conditions

doi: 10.1371/journal.pone.0092120

Figure Lengend Snippet: (A) Quantitative marker analysis showing percent of cells expressing CD73, CD105, CD14 and CD45 after long term expansion Synthemax II microcarriers in Mesencult XF and hMSC stemgro media. Flow cytometry histograms for each phenotypic marker are shown for cells maintained on Synthemax II microcarriers in Mesencult XF medium (B) and hMSC stemgro medium (C).

Article Snippet: Cells were incubated in blocking buffer for 15 min at 4°C, followed by 30 minute incubation at 4°C with primary antibodies against CD14, CD45, CD73, CD105 (Millipore Chemicon) or corresponding isotype controls (Invitrogen/Molecular Probes) in blocking buffer.

Techniques: Marker, Expressing, Flow Cytometry

Journal: Frontiers in Immunology

Article Title: Single-Cell Transcriptomics Revealed Subtype-Specific Tumor Immune Microenvironments in Human Glioblastomas

doi: 10.3389/fimmu.2022.914236

Figure Lengend Snippet: Hypoxia-response TAMs in GBM. (A) UMAP visualization of TAMs. (B) UMAP visualization showing TAMs of individuals. (C) Top 20 function analysis results of TAM-1 cluster. Hypoxia-related biological processes were colored in red. (D) Violin plot of ERO1A expression level in different TAM clusters. (E) Immunofluorescence staining for TAM-1 cluster ( CD14+ERO1A+ ) in patient tumor sample. The staining was performed for seven patients, one section each, and a representative image from patient 6 with TAM-1 pointed out by white arrows was shown; scale, 10 μm. The other images are shown in Supplementary Supplementary Figure 6 . (F) Composition ratio of the TAM clusters in individual GBM. (G) Correlation between the number of MES-like and TAM-1 cells. (H) Heatmap of specific gene sets in TAMs. (I) Violin plot of the hypoxia gene set scores in TAMs. TAM-1 was the hypoxia-response cluster in TAMs. (J) The activated regulons in the TAM-1 cluster ranked by the regulon specificity score from high to low. (K) Survival analysis of the TAM-1 cluster signatures in TCGA and CGGA GBM databases, respectively. Gray dash line, average score; **** p < 0.0001. See also Supplementary Figures 5 and 6 , and Supplementary Tables 3 and 6 .

Article Snippet: The samples were incubated with the first primary antibody against CD14 (1:200 for IF, Servicebio) overnight at 4°C and then with the first corresponding secondary antibody at room temperature for 50 min under dark conditions.

Techniques: Expressing, Immunofluorescence, Staining